anti-calcitonin-gene-related-peptide The dominant search intent for the keyword "anti 2a peptide antibody" is to find and understand antibodies that specifically target the 2A peptide. This includes information on their availability, applications, and specific characteristics.
Here's a breakdown of the entities and phrases:
Tier 1:
* Core Topic: anti 2a peptide antibody
* Key Entity: 2A peptide
* Key Action/Target: antibody
* High-Relevance Phrases: Anti-2A Peptide, 2A Peptide Antibody, anti-2a peptide antibody
Tier 2:
* Variations/Synonyms: 2A peptides, Peptide 2A Antibody, anti-T2A + P2A antibody, anti-T2A antibody, anti-SV2A antibody
* Attributes/Details: clone 3H4, mouse monoclonal antibody, polyclonal rabbit antibody, Western Blot, ICC/IF, Dot Blot, Immunofluorescence, Flow Cytometry, Immunocytochemistry, IP, HRP, Alexa Fluor, BSA Free
* Related Concepts: self-cleaving peptide, polycistronic vectors, protein expression
Tier 3:
* Less Specific/Repetitive: Anti, antibody, antibodies, peptide, 2A, 2A Peptide
* Potentially Off-Topic/Noise: Human Corin Antibody MAB2209-100 (unless contextually linked), other specific antibodies not directly related to 2A peptide.
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Anti-2A peptide antibody is a crucial tool in molecular biology research, enabling scientists to detect and study the presence and function of the 2A peptide作者：Y Ren·2023·被引用次数：5—Typically, a2A peptide, or multiple2A peptides, are ... After electrotransfer, the membrane was incubated with ananti-GFPantibody.... The 2A peptide, a short, self-cleaving peptide sequence derived primarily from viruses like Thosea asigna virus (TAV), plays a vital role in enabling the expression of multiple proteins from a single messenger RNA (mRNA) transcript. This process, known as polycistronic expression, is widely leveraged in genetic engineering and protein production. Antibodies targeting this specific peptide are essential for validating the efficiency of these cleavage events and for tracking the resulting individual proteins.
2A peptides are renowned for their "ribosomal skipping" activity. During translation, the ribosome encounters the 2A sequence and, instead of synthesizing a complete polypeptide chain, it stalls and then restarts translation downstream, effectively cleaving the nascent protein into two separate entitiesArticle MARK, a Novel Family of Protein Kinases That .... This mechanism allows for the simultaneous production of multiple proteins from a single open reading frame (ORF), a technique that simplifies vector design and enhances protein expression efficiency in various systems, including mammalian cells2A). Ovarian cancer cells OVCAR3 and Igrov1 also expressed a similarly sized endogenous protein reactive with theantibody. Following enzymatic deglycosylation, .... Common examples of 2A peptides used in research include those from foot-and-mouth disease virus (FMDV), Thosea asigna virus (TAV), and porcine teschovirus-1 (P2A). The efficiency of this self-cleavage can vary between different 2A sequences and is a critical factor when designing polycistronic constructs.
When selecting an anti-2A peptide antibody, researchers typically encounter monoclonal antibodies, often designated by specific clone numbers such as clone 3H42A peptide-linked polycistronic vectors can be used to express multiple proteins from a single open reading frame (ORF). The small2A peptidesequences, .... These antibodies are frequently raised against synthetic peptides that mimic the conserved C-terminal motif of the 2A peptide sequence, which is typically Asp-Val/Ile-Glu-X-Asn-Pro-Gly-Pro.
Several important characteristics define these antibodies:
* Specificity: The primary goal is high specificity for the 2A peptide sequence, minimizing cross-reactivity with other cellular proteins. Antibodies are often validated for their ability to detect various types of 2A peptides (e.g., T2A, P2A) or are described as "non-species specific."
* Format: Antibodies can be supplied in various formats.作者：P Kręcisz·2020·被引用次数：99—...anti-mouseantibodyproduction effect. Names of humanized Mabs end ... parameters and hematocrit (MPP2A/2B). Successively, measurable ... Some are available as unconjugated primary antibodies, while others are conjugated to reporter molecules like HRP (Horseradish Peroxidase) or fluorescent dyes (e.g., Alexa Fluor® 700, Alexa Fluor® 594, CoraFluor™ 1) for direct detection in assays.
* Validation: Reputable suppliers will provide data validating the antibody's performance in specific research applications. Common validated applications include Western Blot (WB), Immunocytochemistry (ICC/IF), Immunofluorescence (IF), Flow Cytometry (FC), and Immunoprecipitation (IP).
* Purity and Formulation: Some antibodies are offered as "BSA Free," which can be advantageous in certain sensitive assays to avoid potential background interference from bovine serum albumin.
The utility of anti-2A peptide antibodies spans several critical areas of biological research:
* Validation of Polycistronic Expression: The most direct application is confirming that the 2A peptide has successfully mediated self-cleavage in a polycistronic expression systemThis page is the page ofAnti-2A peptide Antibody, clone 3H4 sold by AS ONE CORPORATION. AXEL GLOBAL is a website that lists more than 3 million products .... This is typically done via Western Blotting, where the antibody can detect the cleaved fragments or confirm the absence of the full-length polyprotein.
* Protein Trafficking and Localization Studies: By using fluorescently tagged antibodies or performing immunofluorescence, researchers can study the localization and trafficking of proteins expressed via 2A peptide-mediated systems.
* Gene Therapy and Engineering: In the development of gene therapies or complex genetic circuits, anti-2A peptide antibodies are used to ensure the intended multi-protein expression and cleavage occurs correctlyAnti-2A Peptide Antibody: Cited in 14 publications. All Novus Biologicals antibodies are covered by our 100% guarantee. Scientific Data Images for 2A ....
* Drug Discovery and Development: Understanding protein interactions and expression levels facilitated by 2A peptides can be relevant in screening for potential therapeutic targets or evaluating drug efficacyView2A Peptide Antibody(3H4) [CoraFluor™ 1] (NBP2-59627CL1) validated in Non-species specific & 3 applications from Novus Biologicals..
When choosing an antibody, it is essential to consider the specific 2A peptide sequence intended for detection, as variations may exist. Always consult the manufacturer's datasheet for validated applications, recommended experimental conditions, and potential limitations2A Peptide Antibody (3H4) [Alexa Fluor® 700] from Novus .... For Western Blotting, ensuring the antibody can detect the expected cleavage products or confirm the absence of uncleaved polyproteins is key2023年7月13日—The2A peptideis inserted between the coding sequences of at least two genes, enabling the simultaneous expression of multiple proteins from a .... In imaging applications like immunofluorescence, optimizing antibody concentration and incubation times is crucial to achieve clear and specific staining. The development of recombinant antibodies and antibodies with specific tags further expands the toolkit available to researchers working with 2A peptide-mediated expression systems.
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