sally-hansen-peptide-lip-smoother The SAMS peptide assay is a crucial tool for researchers investigating the activity of AMP-activated protein kinase (AMPK), a key enzyme involved in cellular energy homeostasis and metabolismMore Information. Product Name,SAMS Peptide. Description,SAMS peptideis a substrate for AMP-activated protein kinase (AMPK).. The SAMS peptide itself, with the sequence HMRSAMSGLHLVKRR, serves as a specific synthetic substrate for AMPK. Its utility lies in its ability to be rapidly phosphorylated by AMPK, allowing for the quantification of kinase activity in various experimental settings, particularly within kinase assays. Understanding the SAMS peptide assay is therefore fundamental for studying cellular energy regulation and identifying potential therapeutic targets for metabolic disorders.
AMPK plays a vital role in cellular energy balance, responding to changes in the cellular AMP:ATP ratio. To study its function, researchers often employ in vitro assays to measure its kinase activity. The SAMS peptide, derived from a sequence around Ser79 of rat acetyl-CoA carboxylase, was specifically designed as a selective substrate for mammalian 5'-adenosine monophosphate-activated protein kinase (AMPK). This specificity makes it an ideal tool for precisely measuring AMPK activity, distinguishing it from other protein kinases.
The phosphorylated SAMS peptide can then be detected using various methods, enabling researchers to quantify enzyme activity. This process is essential for screening potential AMPK activators or inhibitors, which are of significant interest for treating conditions like diabetes, obesity, and other metabolic diseases. The development of assays utilizing the SAMS peptide has significantly advanced the study of AMPK signaling pathwaysPeptide-based self-assembled monolayers (SAMs).
Several methodologies can be employed for the SAMS peptide assay, often involving the detection of phosphorylation. The basic principle involves incubating the SAMS peptide with a sample containing AMPK (or purified enzyme) under appropriate reaction conditions. Following the enzymatic reaction, the extent of SAMS peptide phosphorylation is measured.
Common detection methods include:
* Radiometric assays: These historically utilized radiolabeled ATP to detect the transfer of phosphate groups to the SAMS peptideSAMS Peptide [HMRSAMSGLHLVKRR-NH2] - 5 mg.
* Enzyme-linked immunosorbent assays (ELISA): ELISA-based assays can quantify phosphorylated SAMS peptide using specific antibodiesSAMS Peptideis a substrate for AMP-activated protein kinase (AMPK). Phosphorylated rapidly by AMPK, it is more specific for the kinase than acetyl CoA .... This approach offers a non-radioactive and often more sensitive alternative.
* Fluorescence-based assays: Techniques like FRET (Förster Resonance Energy Transfer) or assays using fluorescently labeled antibodies can also be adapted for SAMS peptide phosphorylation detection.
When performing SAMS peptide assays, several factors are critical for obtaining reliable and reproducible results. These include the purity of the SAMS peptide (typically $\ge$95% by HPLC), the concentration of the enzyme and substrate, buffer composition, incubation time, and temperature. The specific formulation of the SAMS peptide, such as whether it is biotinylated or modified for specific detection systems, can also influence the assay design. For instance, biotinylated SAMS peptide can be used in kinase assays involving avidin-biotin detection systems.
The quality and specifications of the SAMS peptide are paramount for the success of any assay. Manufacturers typically provide detailed information including the peptide sequence (HMRSAMSGLHLVKRR-NH2), molecular weight, purity (often $\ge$95% via HPLC), and solubility (usually soluble in water)SAMS Peptideis a substrate for AMP-activated protein kinase (AMPK). Phosphorylated rapidly by AMPK, it is more specific for the kinase than acetyl CoA .... Stability data and recommended storage conditions are also crucial for maintaining peptide integrityEnzo Life Sciences SAMS peptide (biotinylated) (1 mg).
Researchers should also be aware of potential contaminants or residual substances, such as trifluoroacetic acid (TFA), which can sometimes be present from the synthesis process. While TFA typically does not interfere with most standard in vitro assays, it's a consideration for highly sensitive applications. Ensuring the use of high-purity, well-characterized SAMS peptide from reputable suppliers is essential for accurate and consistent AMPK activity measurements.
The SAMS peptide assay is intrinsically linked to broader research in cellular signaling, metabolism, and drug discovery. Its application extends to studying the role of AMPK in:
* Metabolic regulation: AMPK is a central regulator of glucose and lipid metabolism, and its dysregulation is implicated in conditions like type 2 diabetes and metabolic syndromeAnaSpec manufacture and sell custom and catalogpeptides, fluorogenic and FRETassaykits, fluorescent dyes, amino acids, and more for research use, drug ....
* Cancer research: AMPK has been shown to play complex roles in cancer, acting as both a tumor suppressor and, in some contexts, promoting tumor growth.
* Cardiovascular health: AMPK is involved in maintaining cardiac energy homeostasis and has protective effects against cardiovascular diseasesIt is derived from rat acetyl-CoA carboxylase and is known to be a good substrate for AMPK. The biotin allowspeptideto be used in kinaseassayswith ....
* Neuroscience: AMPK signaling in the brain is important for neuronal function and energy balance.
By enabling precise measurement of AMPK activity, the SAMS peptide assay facilitates the discovery and validation of novel therapeutic strategies targeting these diverse physiological and pathological processes.SAMS Peptide The ongoing development of more sensitive and specific assay formats continues to expand the utility of the SAMS peptide in cutting-edge biomedical researchSAMS Peptide - Biochemicals - CAT N°: 17061.
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