Gaussia luciferasesignal peptide The CD33 signal peptide plays a crucial role in the proper targeting and secretion of the CD33 protein. As a key component of the CD33 molecule, this N-terminal sequence directs the nascent polypeptide chain to the endoplasmic reticulum for translocation and subsequent processing, ultimately influencing its function as a myeloid cell surface glycoprotein.Protein-Specific Signal Peptides for Mammalian Vector ... Understanding the CD33 signal peptide is vital for researchers working with recombinant CD33 or developing therapeutic strategies that involve its expressionSignal Peptide Database.
Signal peptides, also known as leader peptides, are short amino acid sequences typically found at the N-terminus of proteins destined for secretion or insertion into cellular membranesWestern blot quantification of secreted SEAP levels using .... Their primary function is to initiate translocation across the endoplasmic reticulum (ER) membraneBelongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. automatic annotation. Once inside the ER, the signal peptide is usually cleaved by a signal peptidase, allowing the mature protein to fold, undergo further modifications, and be directed to its final destination, whether that be secretion out of the cell or integration into various cellular compartmentsORF Clones: CD33, NM_001772.4, G0249. The sequence and properties of a signal peptide can significantly impact the efficiency of protein expression and secretion, making them critical elements in molecular biology and biotechnology.
CD33, also known as myeloid cell surface antigen CD33, is a transmembrane glycoprotein belonging to the Siglec (sialic acid-binding immunoglobulin-like lectin) family. It is predominantly expressed on cells of the myeloid lineage, including monocytes, macrophages, and granulocytes, as well as on leukaemic cells. CD33 plays a role in cell-cell interactions and immune regulation, often acting as an inhibitory receptor. Its specific expression pattern makes it a significant target in research related to hematological malignancies and immune disorders.
The CD33 signal peptide has found practical applications in research, particularly in expression systemsPart:BBa K4829001. For instance, it has been utilized as an N-terminal signal peptide for expressing secreted alkaline phosphatase (SEAP) in HEK293 cells.Overcoming the Refractory Expression of Secreted ... Studies have investigated the impact of the CD33 signal peptide on SEAP expression levels, comparing it to native signal peptides or other commonly used sequences like those from IL-2 or BM40. The effectiveness of the CD33 signal peptide in facilitating secretion has led to its consideration as an industry standard for certain host cells, such as CHO cells, in the development of high-yielding expression systems.
Furthermore, researchers have manipulated the CD33 signal peptide to study its role as a potential binding epitope for antibodies or to assess its influence on protein folding and stability. Variants of CD33 are also associated with diseases like Alzheimer's disease, highlighting the broader biological significance of this molecule and its regulatory sequences. The sequence "MPLLLLLPLLWAGALA" is recognized as a commonly used and effective CD33 signal peptide sequence in laboratory settings due to its brevity and efficiency.
The CD33 signal peptide is an indispensable element for the correct localization and secretion of the CD33 protein. Its role extends beyond simple targeting, influencing expression efficiency in biotechnological applications. As a well-characterized and effective leader sequence, the CD33 signal peptide continues to be a valuable tool in molecular biology research, particularly in the study of myeloid cell function, hematological disorders, and the development of recombinant protein expression systems.
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